153656
Bactericidal/Permeability Increasing Protein (BPI) Recombinant, Human
Size
£346.00
- SKU:
- 153656
- Molecular Weight:
- 39
- Purity:
- ≥95%
- Storage Conditions:
- -70°C
- Supplier:
- United States Biological
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 0.01 sarcosyl, 5% trehalose. Reconstitute with sterile dH2O to 0.1-0.5mg/ml. Do not vortex
- Extra Details:
- Recombinant protein corresponding to Glu140-Thr250 with an N-terminal His and GST Tag expressed in E. coli. Swiss/UniProt Accession: P17213 Purity: ≥95% Amino Acid Sequence: MRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF-E GMSISADLKL GSNPTSGKPT ITCSSCSSHI NSVHVHISKSKVGWLIQLFH KKIESALRNK MNSQVCEKVT NSVSSELQPY FQTLPVMTKI DSVAGINYGL VAPPATTAET Isoelectric Point: 7.3 Predicted Molecular Mass: 41.9kD Accurate Molecular Weight: 42kD (SDS-PAGE reducing conditions) Subcellular Location: Membrane, Secreted Applications: Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -70°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.
- Shipping Conditions:
- Blue Ice