B-Cell Activating Factor (BAFF) Recombinant, Mouse

Size

£326.00

SKU:: 153637
Molecular Weight:: 27.7
Purity:: ≥95%
Storage Conditions:: -70°C
Supplier:: United States Biological
Format:: Supplied as lyophilized powder from 20mM Tris, 150mM sodium chloride, pH 8.0, 1mM EDTA, 1mM DTT, 5% trehalose, 0.01% sarcosyl, 0.05% Proclin-300. Reconstitute with sterile 20mM Tris, 150mM sodium chloride, pH 8.0 to a concentration of 0.1-1mg/ml. Do not vortex.
Extra Details:: Recombinant protein corresponding to Tyr68-Phe283 with an N-terminal His-Tag from B-Cell Activating Factor expressed in E. coli. (Q9WU72) Purity: ≥95% Amino Acid Sequence: YQLAALQADL MNLRMELQSY RGSATPAAAG APELTAGVKL LTPAAPRPHN SSRGHRNRRA FQGPEETEQD VDLSAPPAPC LPGCRHSQHD DNGMNLRNII QDCLQLIADS DTPTIRKGTY TFVPWLLSFK RGNALEEKEN KIVVRQTGYF FIYSQVLYTD PIFAMGHVIQ RKKVHVFGDE LSLVTLFRCI QNMPKTLPNN SCYSAGIARL EEGDEIQLAI PRENAQISRN GDDTF Predicted Isoelectric Point: 7.9 Predicted Molecular Mass: 24.3kD Accurate Molecular Weight: 32kD (SDS-PAGE reducing conditions) Note: The possible reasons that the actual band size differs from the predicted are as follows: 1. Splice variants: Alternative splicing may create different sized proteins from the same gene 2. Relative charge: The composition of amino acids may affect the charge of the protein. 3. Post-translational modification: Phosphorylation, glycosylation, methylation etc. 4. Post-translation cleavage: Many proteins are synthesized as pro-proteins and then cleaved to give the active form. 5. Polymerization of the target protein: Dimerization, multimerization etc. Subcellular Location: Membrane Applications: Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -70°C. Stable for 6 months after receipt. Reconstitute with sterile 20mM Tris, 150mM sodium chloride, pH 8.0. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.

Shipping Conditions:: Blue Ice