Aspartate Aminotransferase (AST) Recombinant, Rat

Size

£384.00

SKU:: 153615
Molecular Weight:: 47.7
Purity:: ≥95%
Storage Conditions:: 4°C/-70°C
Supplier:: United States Biological
Format:: Supplied as lyophilized powder from 20mM Tris, 150mM sodium chloride, pH 8.0, 1mM EDTA, 1mM DTT, 5% trehalose, 0.01% sarcosyl, 0.05% Proclin-300. Reconstitute with sterile 20mM Tris, 150mM sodium chloride, pH 8.0 to a concentration of 0.1-1mg/ml. Do not vortex.
Extra Details:: Source: Recombinant Rat from E. coli Accession No: P13221 Fragment: Met1~Gln413 (Accession No: P13221) Sequence: MGHHHHHHSGS-MAPPSFFAQV PQAPPVLVFK LIADFRDDPD PRKVNLGVGA YRTDDSQPWV LPVVRKVEQK IANDHSLNHE YLPILGLAEF RSCASQLVLG DNSPALRENR VGGVQSLGGT GALRIGADFL GRWYNGTDNK NTPVYVSSPT WENHNGVFSA AGFKDIRSYR YWDAEKRGLD LQGFLNDLEN APEFSIFVLH ACAHNPTGTD PTEEEWKQIA AVMKRRFLFP FFDSAYQGFA SGDLEKDAWA IRYFVSEGFE LFCAQSFSKN FGLYNERVGN LTVVGKEHDS VLRVLSQMEK IVRITWSNPP AQGARIVATT LSNPELFKEW KGNVKTMADR ILTMRSELRA RLEALKTPGT WSHITEQIGM FSFTGLNPKQ VEYLVNEKHI YLMPSGRINM CGLTTKNLDY VATSINEAVT KFQ Epitope Tag: N-terminal His-Tag Molecular Weight: 47.7kD Applications: Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -70°C. Reconstitute with sterile 20mM Tris, 150mM sodium chloride, pH 8.0.. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.

Shipping Conditions:: Blue Ice