153461
Amylase Alpha 2, Pancreatic (AMY2) Recombinant, Rat
Size
£355.00
- SKU:
- 153461
- Molecular Weight:
- 34.4
- Purity:
- ≥80%
- Storage Conditions:
- -70°C
- Supplier:
- United States Biological
- Format:
- Supplied as lyophilized powder from PBS, pH 7.4, 0.01% sarcosyl, 5% trehalose. Reconstitute with ddH2O to a concentration of 0.1-0.2mg/ml. Do not vortex.
- Extra Details:
- Recombinant protein corresponding to aa206-478 with an N-Terminal His Tag from rat AMY2, expressed in E. coli. Uniprot/Accession: G3V844 Amino Acid Sequence: IGVAGFRLDA AKHMWPGDIK AVLDKLHNLN TKWFSQGSRP FIFQEVIDLG GEAIKGSEYF GNGRVTEFKY GAKLGTVIRK WNGEKMSYLK NWGEGWGFVP TDRALVFVDN HDNQRGHGAG GASILTFWDA RMYKMAVGFM LAHPYGFTRV MSSYRWARNF QNGKDVNDWI GPPNNNGVTK EVTINPDTTC GNDWVCEHRW RQIRNMVAFR NVVNGQPFAN WWDNGSNQVA FSRGNRGFIV FNNDDWALSS TLQTGLPAGT YCDVISGDKV NGN Predicted Isoelectric Point: 9.3 Predicted Molecular Weight: 34.4kD Accurate Molecular Mass: 34kD as determined by SDS-PAGE reducing conditions. Subcellular Location: Secreted Applications: Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -70°C. Reconstitute with ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.
- Shipping Conditions:
- Blue Ice