Alpha-1-Microglobulin/Bikunin Precursor (a1M) Recombinant, Rat

Size

£341.00

SKU:: 153443
Molecular Weight:: 50.8
Purity:: ≥90%
Storage Conditions:: -70°C
Supplier:: United States Biological
Format:: Supplied as lyophilized powder from 20mM Tris, 150mM sodium chloride, pH 8.0, 0.01% sarcosyl, 5% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml. Do not vortex.
Extra Details:: Recombinant protien corresponding to Asp20-Ala202 with an N-Terminal His and GST Taqg from rat a1M, expressed in E. coli. Swiss/UniProt Accession: Q64240 Amino Acid Sequence: D NVPTLPDIQV QENFNEARIY GKWFNLAVGS TCPWLRRIKN KMSVSTLVLQ EGATEAEISV TSTQWRKGVC EEISGVYQKT DIDGKFLYHK SKWNATLESY VVHTNYDEYA IFLTKKFSHR HGPTITAKLY GREPQLRDSL LQEFREVALS VGIPENSIVF MADRGECVPG DREVESTSFA RA Predicted Isoelectric Point: 6.4 Predicted Molecular Weight: 50.8kD Accurate Molecular Weight: 50kD as determined by SDS-PAGE reducing conditions Subcellular Location: Secreted Applications: Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -70°C. Stable for 6 months after receipt. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.

Shipping Conditions:: Blue Ice