Alpha-1-Microglobulin/Bikunin Precursor (a1M) Recombinant, Rat

Size

£341.00

SKU:: 153442
Molecular Weight:: 22.4
Purity:: ~90% Endotoxin:≤1EU/ug (LAL)
Storage Conditions:: 4°C/-70°C
Supplier:: United States Biological
Format:: Supplied as lyophilized powder from PBS, pH 7.4, 1mM DTT, 5% trehalose, 0.01% sarcosyl, 0.05% Proclin-300. Reconstitute with sterile PBS to a concentration of 0.1-1mg/ml. Do not vortex.
Extra Details:: Recombinant protein corresponding to aa20-202 of rat a1M with N-terminal His-tag expressed in E. coli (Q64240). AA Sequence: MGHHHHHHSGSEF-D NVPTLPDIQV QENFNEARIY GKWFNLAVGS TCPWLRRIKN KMSVSTLVLQ EGATEAEISV TSTQWRKGVC EEISGVYQKT DIDGKFLYHK SKWNATLESY VVHTNYDEYA IFLTKKFSHR HGPTITAKLY GREPQLRDSL LQEFREVALS VGIPENSIVF MADRGECVPG DREVESTSFA RA Predicted Molecular Weight: 22.4kD Subcellular Location: Secreted Predicted isoelectric point: 6.4 Applications: Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -70°C. Stable for 6 months after receipt at -70°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Included Protein Molecular Weight Marker: 168631: Protein Molecular Weight Marker, Unstained Ready to use, No heating, diluting or reducing agents needed. MW: 10kD, 14kD, 18kD, 22kD, 26kD, 33kD, 44kD, 70kD Double intensity: 10kD, 18kD, 26kD Supplied in 62.5mM Tris-H3PO4, pH 7. 1mM EDTA, 2% SDS, 100mM DTT, 1m sodium azide, 0.01% bromo-phenol blue, 33% glycerol. Add 3-5ul/well for mini gels; 7ul/well for large gels. Store at -20 Stable for 6 months after receipt. Note: Thermal stability is described by the los rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37ºC for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions

Shipping Conditions:: Blue Ice