ITK (Tyrosine-protein Kinase ITK/TSK, Interleukin-2-inducible T Cell Kinase, IL-2-inducible T Cell Kinase, Kinase EMT, T-cell-specific Kinase, Tyrosine-protein Kinase Lyk, EMT, LYK, MGC126257, MGC126258)

Size

£696.00

SKU:: 150573
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Synthetic peptide corresponding to C-RLLRQLAEIAESGL, from the C-terminal of human ITK. Species sequence homology: mouse
Format:: Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Extra Details:: Applications: Suitable for use in ELISA, Immunofluorescence and Western Blot. Other applications not tested. Recommended Dilutions: Peptide ELISA: 1:2000 Western Blot: 1-3ug/ml. ~75kD band observed in nuclear lysates of cell line Jurkat (calculated MW of 71.8kD) Immunofluorescence: 10ug/ml. Strong expression of the protein seen in the cytoplasm of HeLa and Jurkat cells. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.