150016
CD14 (Monocyte Differentiation Antigen CD14, Myeloid Cell-specific Leucine-rich Glycoprotein)
Size
£709.00
- SKU:
- 150016
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Goat
- Immunogen:
- Synthetic peptide corresponding to 14aa, C-KRVDADADPRQYAD, from near the internal region of human CD14.
- Format:
- Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
- Extra Details:
- CD14 acts as a receptor for endotoxin (LPS), is expressed strongly on monocy¬tes, macrophages and weekly at surface of neutrophiles. CD14 is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor LPS-LBP (lipopolysaccharide binding protein)-complexes. Furthermore CD14 is present in a soluble form in human serum, urine and other body fluids, acts at physiological concentrations as LPS agonist and has at higher concentrations an antagonizing effect in cell activation. The myeloid differentiation antigen CD14 acts as the major receptor for bacterial LPS. The dominant form of the recombinant wild type CD14 is the 50kD protein. This is a His-tagged protein. Applications: Suitable for use in ELISA, Western Blot and Flow Cytometry. Other applications not tested. Recommended Dilutions: ELISA: 1:128,000 Western Blot: 1-3ug/ml Human lymph nodes, tonsil lysates Flow Cytometry: 10ug/ml A549 cells Optimal dilutions to be determined by the researcher. Positive Control: Human Lymph Node Tissue Lysate Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice