Calreticulin, Recombinant, aa18-417, His-Tag (CRP55, Calregulin, Endoplasmic Reticulum Resident Protein 60, ERp60, HACBP, grp60, CRTC, CALR)

Size

£992.00

SKU:: 149797
Molecular Weight:: 46
Purity:: ~90%
Storage Conditions:: -70°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 10mM Tris, pH 8.0, 0.002% sodium azide, 3mM sodium chloride.
Extra Details:: Calreticulin is a multifunctional protein that acts as a major Ca(2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. It is also found in the nucleus, suggesting that it may have a role in transcription regulation. Calreticulin binds to the synthetic peptide KLGFFKR, which is almost identical to an aa sequence in the DNA-binding domain of the superfamily of nuclear receptors. Calreticulin binds to antibodies in certain sera of systemic lupus and Sjogren patients which contain anti-Ro/SSA antibodies, it is highly conserved among species, and it is located in the endoplasmic and sarcoplasmic reticulum where it may bind calcium. Source: Recombinant protein corresponding to CALR, fused to His-tag at N-terminal expressed in E.coli. Molecular Weight: ~46kD Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.