149757
sFas Ligand, Recombinant, Human, His-Tag
Size
£351.00
- SKU:
- 149757
- Molecular Weight:
- 17.9
- Purity:
- ≥95% (SDS-PAGE and HPLC). Endotoxin: ≤0.1ng/ug (1EU/ug)
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Format:
- Supplied as a lyophilized powder. Reconstitute with sterile ddH2O to 0.1-1mg/ml
- Extra Details:
- Fas Ligand (FasL) is a member of the TNF superfamily that is expressed on the cell surface of activated T cells. Binding of FasL to Fas Receptor triggers apoptosis in Fas-bearing cells. FasL has the ability to kill T cells and activated B cells, which leads to down-regulation of the immune response. The mechanism of Fas-induced apoptosis involves recruitment of pro-caspase 8 through an adaptor molecule called FADD, followed by processing of the pro-enzyme into active forms. These active caspases then cleave various cellular substrates, leading to the eventual cell death. Both human and murine sFasL are fully active on human and murine cells. Recombinant Human soluble Fas Ligand is a 17.9kD protein comprising the TNF-homologous region of FasL and contains an 8-residue N-terminal His-Tag. Recombinant protein corresponding to human sFas Ligand, fused to a 8 residue N-terminal His-Tag, expressed in CHO cells. Accession Number: NP_000630.1 Biological Activity: Determined by its ability to induce cytotoxicity in Jurkat cells in the absence of any cross-linking. The ED50 for this effect is ≤10ng/ml, corresponding to a specific activity of ≥1x10e5 units/mg. Amino Acid Sequence: HHHHHHHHPS PPPEKKELRK VAHLTGKSNS RSMPLEWEDT YGIVLLSGVK YKKGGLVINE TGLYFVYSKV YFRGQSCNNL PLSHKVYMRN SKYPQDLVMM EGKMMSYCTT GQMWARSSYL GAVFNLTSAD HLYVNVSELS LVNFEESQTF FGLYKL Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice