Interleukin 6, Recombinant, Human (Interleukin-6, IL6, IL-6, B Cell Stimulatory Factor 2, BSF2, BSF-2, CTL Differentiation Factor, CDF, Hybridoma Growth Factor, HGF, HSF, Interferon beta-2, IFN-beta-2, IFNB2)

Size

£509.00

SKU:: 149749
Purity:: ≥98% by SDS-PAGE and HPLC. Endotoxin: ≤0.1ng/ug.
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Reconstitute with sterile ddH2O to a concentration of 0.1-0.5mg/ml. Gently shake the solution for about 10 minutes for better solubility. Do not vortex.
Extra Details:: IL-6 is a pleiotropic cytokine that plays an important role in host defense by regulating immune and inflammatory responses. Produced by T cells, monocytes, fibroblasts, endothelial cells and keratinocytes, IL-6 has diverse biological functions. It stimulates B-cell differentiation and antibody production, synergizes with IL-3 in megakaryocyte development and platelet production, induces expression of hepatic acute-phase proteins, and regulates bone metabolism. IL-6 signals through the IL-6 receptor system that consists of two chains, IL-6Rα and gp130. Murine IL-6 is inactive on human cells, while both human and murine are equally active on murine cells. Recombinant human IL-6 is a 20.9kD protein containing 184aa. Manufactured using all non-animal reagents. Source: Recombinant protein corresponding to human IL-6, expressed in E.coli. Biological Activity: Determined by the dose-dependent stimulation of the proliferation of the IL-6 dependent murine 7TD1 cells. The ED50 was found to be 0.1ng/ml Specific Activity: 1x10e7units/mg Endotoxin: <0.1ng/ug (1EU/ug) AA Sequence: PVPPGEDSKD VAAPHRQPLT SSERIDKQIR YILDGISALR KETCNKSNMC ESSKEALAEN NLNLPKMAEK DGCFQSGFNE ETCLVKIITG LLEFEVYLEY LQNRFESSEE QARAVQMSTK VLIQFLQKKA KNLDAITTPD PTTNASLLTK LQAQNQWLQD MTTHLILRSF KEFLQSSLRA LRQM Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice