Nerve Growth Factor, beta, Recombinant, Human (Beta-nerve Growth Factor, Beta-NGF, NGFb, NGF, HSAN5)

Size

£397.00

SKU:: 149732
Molecular Weight:: 27
Purity:: ≥98% (SDS-PAGE and HPLC). Endotoxin: ≤0.1ng/ug (1EU/ug)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a lyophilized powder. Reconstitute with sterile ddH2O to 0.1-1mg/ml.
Extra Details:: β-NGF is a neurotrophic factor structurally related to BDNF, NT-3 and NT-4. These proteins belong to the cysteine-knot family of growth factors that assume stable dimeric structures. β-NGF is a potent neurotrophic factor that signals through its receptor β-NGFR, and plays a crucial role in the development and preservation of the sensory and sympathetic nervous systems. β-NGF also acts as a growth and differentiation factor for B lymphocytes, and enhances B-cell survival. The functional form of Recombinant human β-NGF is a non-covalently-linked homodimer of two 13.5kD, polypeptide monomers that each contain 120 amino acids and three disulfide bonds, which are required for biological activity. Recombinant protein corresponding to human NGF beta, expressed in E. coli. Biological Activity: Determined by the dose-dependent stimulation of the proliferation of human TF-1 cells. The expected ED50 is ≤ 1ng/ml, corresponding to a specific activity of ≥1x10e6 units/mg. Amino Acid Sequence: SSSHPIFHRG EFSVCDSVSV WVGDKTTATD IKGKEVMVLG EVNINNSVFK QYFFETKCRD PNPVDSGCRG IDSKHWNSYC TTTHTFVKAL TMDGKQAAWR FIRIDTACVC VLSRKAVRRA Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice