sOX40 Ligand, Recombinant, Human (TTNFSF4, OX40L, Glycoprotein Gp34, CD252, TXGP1)

Size

£351.00

SKU:: 149661
Purity:: ≥97% (SDS-PAGE and HPLC). Endotoxin: <0.1ng/ug (1EU/ug)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a lyophilized powder from 10mM Tris pH 8.5. Reconstitute with sterile ddH2O to 0.5mg/ml.
Extra Details:: The tumor necrosis factor superfamily member TNFSF4 is a type II membrane bound, non-covalently linked homotrimeric protein. It is expressed on antigen presenting cells, such as dendritic cells and activated B-cells, and also on other cells such as vascular endothelial cells, mast cells, and natural killer cells. TNFSF4 signals specifically through the TNFRSF4 receptor, is expressed predominantly on CD4+T cells but also on certain activated CD8+T cells. TNFRSF4/TNFSF4 functions as a costimulatory signal, which is required for a productive interaction between antigen presenting cells and their target T-cells. It enhances cell proliferation and survival, and increases expression of RANTES, IL-2, IL-3, and IFNgamma. TNFRSF4/TNFSF4 signaling plays an important role in immunotolerance. Recombinant OX40L is a glycosylated 133 amino acid protein corresponding to the extracellular TNF homologous domain of the full length transmembrane protein. It migrates with an apparent molecular mass of 15.5-25kD on SDS-PAGE. Recombinant protein corresponding to human OX40 Ligand, expressed in Hi-5 Insect cells. Biological Activity: Determined by its ability to bind hOX40 in a functional ELISA. Amino Acid Sequence: QVSHRYPRIQ SIKVQFTEYK KEKGFILTSQ KEDEIMKVQN NSVIINCDGF YLISLKGYFS QEVNISLHYQ KDEEPLFQLK KVRSVNSLMV ASLTYKDKVY LNVTTDNTSL DDFHVNGGEL ILIHQNPGEF CVL Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice