Trefoil Factor 3, Recombinant, Human (TFF3, Intestinal Trefoil Factor, hITF, Polypeptide P1.B, hP1.B, ITF, TFI)

Size

£341.00

SKU:: 149649
Purity:: ~98% (SDS-PAGE and HPLC)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a lyophilized powder.
Extra Details:: The Trefoil Factor peptides (TFF1, TFF2 and TFF3) are secreted in the gastrointestinal tract, and appear to play an important role in intestinal mucosal defense and repair. TFF-3 is expressed by goblet cells and in the uterus, and has also been shown to express in certain cancers, including colorectal, hepatocellular, and in biliary tumors. TFF3 may be useful as a molecular marker for certain types of cancer, but its role, if any, in tumorigenesis is unknown. TFF3 also promotes airway epithelial cell migration and differentiation. Recombinant human TFF3 is a 13.2kD homodimeric protein consisting of two 59aa chains, which includes a 40aa trefoil motif containing three conserved intramolecular disulfide bonds. Source: Recombinant protein corresponding to human Trefoil Factor 3, expressed in E.coli. Biological Activity: Determined by its ability to chemoattract human MCF-7 cells using a concentration range of 1-10ug/ml. Endotoxin: <0.1ng/ug (1EU/ug) AA Sequence: EEYVGLSANQ CAVPAKDRVD CGYPHVTPKE CNNRGCCFDS RIPGVPWCFK PLQEAECTF Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice