GDF3, Recombinant, Human (Growth/Differentiation Factor 3, GDF-3, UNQ222/PRO248, KFS3, MCOP7, MCOPCB6)

Size

£341.00

SKU:: 149611
Purity:: ~98% (SDS-PAGE and HPLC)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a lyophilized powder.
Extra Details:: GDF-3 is a member of the TGF-beta superfamily of growth and differentiation factors, and is highly homologous to GDF-9. Unlike most TGF-beta family members, GDF-3 and GDF-9 are not disulfide-linked dimers. GDF-3 is expressed in adult bone marrow, spleen, thymus, and adipose tissue. The expression of GDF-3 is upregulated in high-fat-fed wild-type FABP4/aP2 null mice and was associated with obesity, but not with the related hyperglycemia/hyperinsulinemia which characterizes Type 2 diabetes. Recombinant human GDF-3 is a 26kD non-disulfide-linked homodimer containing two 114aa polypeptide chains. Source: Recombinant protein corresponding to human GDF-3, expressed in E.coli. Biological Activity: Determined by its ability to inhibit induced alkaline phosphatase production by ATDC-5 chondrogenic cells. The ED50 for this effect is 100-150ng/ml. Endotoxin: <0.1ng/ug (1EU/ug) AA Sequence: AAIPVPKLSC KNLCHRHQLF INFRDLGWHK WIIAPKGFMA NYCHGECPFS LTISLNSSNY AFMQALMHAV DPEIPQAVCI PTKLSPISML YQDNNDNVIL RHYEDMVVDE CGCG Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.