VEGFC, Recombinant, Human (Vascular Endothelial Growth Factor C, VEGF-C, Flt4 ligand, Flt4-L, Vascular Endothelial Growth Factor-related Protein, VRP)

Size

£364.00

SKU:: 149583
Purity:: ≥95% (SDS-PAGE and HPLC). Endotoxin: ≤0.1ng/ug (<1EU/ug).
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a lyophilized powder. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml. Do not raise to neutral pH and do not vortex.
Extra Details:: VEGF-C, a member of the VEGF/PDGF family of structurally related proteins, is a potent angiogenic cytokine. It promotes endothelial cell growth, promotes lymphangiogesis, and can also affect vascular permeability. VEGF-C is expressed in various tissues, but is not produced in peripheral blood lymphocytes. It forms cell surfaced-associated, non-covalent, disulfide-linked homodimers, and can bind and activate both VEGFR-2 (flk1) and VEGFR-3 (flt4) receptors. During embryogenesis, VEGF-C may play a role in the formation of the venous and lymphatic vascular systems. Both VEGF-C and VEGF-D are over-expressed in certain cancers, and the resulting elevated levels of VEGF-C or VEGF-D tend to correlate with increased lymphatic metastasis. Recombinant human VEGF-C is a disulfide-linked homodimeric protein consisting of two 13.5kD polypeptide chains of 116 amino acid residues. Due to glycosylation, the protein migrates as a 30-33kD band by SDS-PAGE analysis under non-reducing conditions. Recombinant protein corresponding to human VEGF-C, expressed in HEK 293. Molecular Weight: ~26.2kD (calculated) Biological Activity: Determined by its ability to stimulate the proliferation of human microvascular endothelial cells (HMVEC) in low serum conditions. Amino Acid Sequence: AHYNTEILKS IDNEWRKTQC MPREVCIDVG KEFGVATNTF FKPPCVSVYR CGGCCNSEGL QCMNTSTSYL SKTLFEITVP LSQGPKPVTI SFANHTSCRC MSKLDVYRQV HSIIRR Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice