VEGF121, Recombinant, Human (Vascular Endothelial Growth Factor121, VPF)

Size

£351.00

SKU:: 149581
Molecular Weight:: 28.4
Purity:: ≥98% (SDS-PAGE and HPLC). Endotoxin: ≤0.1ng/ug (1EU/ug)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a lyophilized powder in 10mM sodium phosphate, pH 7.5. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml. Do not Vortex.
Extra Details:: VEGF is a potent growth and angiogenic cytokine. It stimulates proliferation and survival of endothelial cells, and promotes angiogenesis and vascular permeability. Expressed in vascularized tissues, VEGF plays a prominent role in normal and pathological angiogenesis. VEGF signals through three receptors; fms-like tyrosine kinase (flt-1), KDR gene product (the murine homolog of KDR is the flk-1 gene product) and the flt4 gene product. Due to its increased acidity, VEGF121 circulates more freely than other VEGF forms, which bind more tightly with vascular heparin sulfates. Recombinant Human VEGF121 is a 28.4kD, disulfide-linked homodimeric protein consisting of two 121 amino acid polypeptide chains. Recombinant protein corresponding to human VEGF121, expressed in E. coli. Accession/Uniprot: P15692 Molecular Weight: ~28.4kD Biological Activity: Determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC) using a concentration range of 0.2-0.4ng/ml. Amino Acid Sequence: APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQENCDKPR R Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice