PION (GSAP, Gamma-secretase-activating Protein, Protein Pigeon Homolog, DKFZp667B242, LOC54103, MGC126548)

Size

£655.00

SKU:: 146793
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to 19aa from near the C-terminus of human PION.
Format:: Supplied as a liquid in PBS, 0.02% sodium azide.
Extra Details:: Accumulation of the amyloid-B peptide (AB) in the cerebral cortex is a critical event in the pathogenesis of Alzheimers disease. The Bamyloid protein precursor (APP) is cleaved by one of two Bsecretases (BACE and BACE2), producing a soluble derivative of the protein and a membrane anchored 99aa carboxy-terminal fragment (C99). The C99 fragment serves as substrate for ysecretase to generate the 4kD amyloid-B peptide (AB), which is deposited in the Alzheimers disease patients brains. PION, or GSAP, selectively increases amyloid-beta production through a mechanism involving its interaction with both gamma-secretase and the APP C-terminal fragment, suggesting that PION may be a potential therapeutic target for the treatment of Alzheimer's disease. Applications: Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.25ug/ml Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml Optimal dilutions to be determined by the researcher. Positive Control: EL4 Cell Lysate Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice