DCLK3 (Serine/Threonine-protein Kinase DCLK3, Doublecortin Domain-containing Protein 3C, Doublecortin-like and CAM Kinase-like 3, Doublecortin-like Kinase 3, DCAMKL3, DCDC3C, KIAA1765)

Size

£655.00

SKU:: 146611
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to 14aa from near the N-terminus of human DCLK3. Epitope: within the last 50 amino acids of DCLK3.
Format:: Supplied as a liquid in PBS, 0.02% sodium azide.
Extra Details:: DCLK3 is one of three doublecortin-like kinases similar to the Ca2+/calmodulin-dependent protein kinase (CaMK) family. DCLK3 mRNA, like that of the homologous DCLK1 and DCLK3, is highly expressed in adult brain, but only DCLK3 transcripts are present in liver and kidney, suggesting that DCLK3 may play other roles than in cortical development. The DCLK proteins are homologous to Doublecortin (DCX), a protein that is mutated in X-linked human lissencephaly. In mouse models where the DCX gene has been disrupted, DCLK1 expression increases slightly and appears to compensate for the loss of DCX, as mice mutant for both DCX and DCLK1 show a severe phenotype including perinatal lethality, disorganized neocortical layering, and profound hippocampal cytoarchitectural disorganization. Applications: Suitable for use in Immunofluorescence, ELISA, Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1-2ug/ml Immunohistochemistry (paraffin): 5ug/ml Immunofluorescence: 20ug/ml Optimal dilutions to be determined by the researcher. Positive Control: K562 Cell Lysate Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice