146522
RUSC1 (RUN and SH3 Domain-containing Protein 1, New Molecule Containing SH3 at the Carboxy-terminus, NESCA, DKFZp761A1822)
Size
£655.00
- SKU:
- 146522
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to 17aa from near the C-terminus of human RUSC1.
- Format:
- Supplied as a liquid in PBS, 0.02% sodium azide.
- Extra Details:
- RUSC1, also known as NESCA, shares with the related protein RUSC2 a common domain structure of RUN, leucine zipper and SH3 domain in addition to over 30% aa identity. RUSC1 is an adapter protein that can bind to the TrkA receptor and is necessary in the NGF-induced neurite growth of PC12 cells. RUSC1 has also been shown to interact with IkappaB kinase- (IKK-) gamma, the regulatory subunit of the IKK complex that is required for NF-kappaB activation in many signaling pathways such as TNF-R or the TLR pathways. RUSC1 can also bind to the E3 ubiquitin ligase TRAF6, which then catalyzes RUSC1 polyubiquitination. Since overexpression of RUSC1 strongly inhibits TRAF6-mediated polyubiquitination of IKK-gamma, RUSC1 may be a link in the IKK-gamma-mediated NF-kappaB activation pathway. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher. Positive Control: A20 Cell Lysate Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice