AP2M1 (CLAPM1, KIAA0109, AP-2 Complex Subunit mu, AP-2 mu Chain, Adapter-related Protein Complex 2 mu Subunit, Adaptin-mu2, Adaptor Protein Complex AP-2 Subunit mu, Clathrin Assembly Protein Complex 2 Medium Chain, Clathrin Coat Assembly Protein AP50, Clathrin Coat-associated Protein AP50, HA2 50kD Subunit, Plasma Membrane Adaptor AP-2 50kD Protein)

Size

£655.00

SKU:: 146439
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Chicken/Avian
Immunogen:: Synthetic peptide corresponding to 16aa from near the C-terminus of human AP2M1.
Format:: Supplied as a liquid in PBS, 0.02% sodium azide.
Extra Details:: The heterotetrameric coat assembly protein complex, also known as the adaptor-related protein complex 2 (AP-2), belongs to the adaptor complexes medium subunits family. The mu 1 subunit of the AP-2 complex (AP2M1) is required for the activity of a vacuolar ATPase, which is responsible for proton pumping occurring in the acidification of endosomes and lysosomes. AP2M1 has also been shown to associate with the HIV-1 protein Nef, suggesting that Nef may use AP-2 complex to enhance the rate of endocytosis of both CD4 and class I MHC. AP2M1 may also play an important role in regulating the intracellular trafficking and function of cytotoxic T-lymphocyte associated (CTLA)-4 protein. At least two isoforms of AP2M1 are known to exist. Applications: Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1-2ug/ml Immunohistochemistry (Formalin fixed paraffin embedded): 2.5ug/ml Immunofluorescence: 20ug/ml Optimal dilutions to be determined by the researcher. Positive Control: Human Kidney Tissue Lysate Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice