146226
SLC9A1, CT (Sodium/hydrogen Exchanger 1, Na(+)/H(+) Exchanger 1, NHE-1, Solute Carrier Family 9 Member 1, Na(+)/H(+) Antiporter, Amiloride-sensitive, APNH, APNH1, NHE1)
Size
£655.00
- SKU:
- 146226
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to 20aa from near the C-terminus of human Nhe-1. Epitope: within the last 50aa of the protein
- Format:
- Supplied as a liquid in PBS, 0.02% sodium azide.
- Extra Details:
- The Na+/H+ antiporter (Nhe-1) is a ubiquitous membrane-bound enzyme involved in pH regulation of vertebrate cells and is specifically inhibited by the diuretic drug amiloride and activated by a variety of signals including growth factors, mitogens, neurotransmitters, and tumor promoters. Nhe-1 acts as an anchor for actin filaments to control the integrity of the cortical cytoskeleton. This occurs through a previously unrecognized structural link between Nhe-1 and the actin-binding proteins ezrin, radixin, and moesin, collectively referred to as ERM proteins. A structural role for Nhe-1 has been proposed in regulating the cortical cytoskeleton that is independent of its function as an ion exchanger. It is also thought that Nhe-1 play a role in hypertension. At least two isoforms of Nhe-1 are known to exist. Applications: Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1-2ug/ml Immunofluorescence: 20ug/ml Immunohistochemistry (FFPE): 2.5ug/ml Optimal dilutions to be determined by the researcher. Positive Control: Rat Kidney Tissue Lysate, Human Kidney Tissue Lysate Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice