146144
TMED10, ID (Transmembrane emp24 Domain-containing Protein 10, 21kD Transmembrane-trafficking Protein, S31III125, S31I125, Tmp-21-I, Transmembrane Protein Tmp21, p23, p24 Family Protein delta-1, p24delta, TMP21)
Size
£655.00
- SKU:
- 146144
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to 18aa from human TMP21.
- Format:
- Supplied as a liquid in PBS, 0.02% sodium azide.
- Extra Details:
- TMP21 is a ubiquitously expressed protein that is involved in vesicular targeting and protein transport. More recent experiments have shown that it is also a component in the presenilin complex and modulates the y-secretase but not the esecretase cleavage activity of the amyloid precursor protein. The presenilin complex is composed of the proteins APH1, nicastrin, and PEN2 in addition to presenilin-1. Together, these proteins cleave the amyloid precursor protein at what is known as the y and e-sites and can lead to the accumulation of the Ab cleavage product that is associated with Alzheimer's disease. Co-immunoprecipitation experiments using antibodies against these proteins also yielded TMP21 indicating that TMP21 may play a role in the regulation of this complex. Suppression of TMP21 expression by siRNA in transfected cells caused increased y-secretase activity but not e-secretase activity, and increased Ab production, demonstrating that TMP21 can modulate ysecretase activity. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.5-2ug/ml Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml Optimal dilutions to be determined by the researcher. Positive Control: Mouse Brain Tissue Lysate or Human Brain Tissue Lysate Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice