PEN2, CT (PSENEN, Presenilin Enhancer Protein 2, Gamma-secretase Subunit PEN-2, MDS033, MSTP064)

Size

£655.00

SKU:: 146135
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to 13aa from near the C-terminus of human PEN2.
Format:: Supplied as a liquid in PBS, 0.02% sodium azide.
Extra Details:: PEN2, in addition to presenilin, nicastrin, and APH-1 forms the y-secretase protein complex, a membrane-bound aspartyl protease that can cleave certain proteins at peptide bonds buried within the hydrophobic environment of the lipid bilayer. This cleavage is responsible for a key step in signaling from several cell-surface receptors and is thought to be required for the generation of the neurotoxic amyloid peptides that are central to the pathogenesis of Alzheimer’s disease. Like the tumor necrosis factor-a-converting enzyme (TACE) and the b-site cleavage enzyme (BACE) protease families, y-secretase will cleave the amyloid precursor protein (APP), but within the intramembrane region of APP, resulting in either the non-toxic p3 (from the a and y cleavage site) or the toxic Ab amyloid peptide (from the b and y cleavage site). It is thought that accumulation of the Ab peptide is the precursor to Alzheimer's disease. Applications: Suitable for use in Immunofluorescence, ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.5-2ug/ml Immunohistochemistry (Formalin fixed paraffin embedded): 20ug/ml Optimal dilutions to be determined by the researcher. Positive Control: K562 Cell Lysate Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice