MTOR (Serine/Threonine-protein Kinase mTOR, FK506-binding Protein 12-rapamycin Complex-associated Protein 1, FKBP12-rapamycin Complex-associated Protein, Mammalian Target of Rapamycin, mTOR, Mechanistic Target of Rapamycin, Rapamycin and FKBP12 Target 1, Rapamycin Target Protein 1, FRAP, FRAP1, FRAP2, RAFT1, RAPT1, FLJ44809)

Size

£655.00

SKU:: 146047
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to 15aa from near the N-terminus of human TOR.
Format:: Supplied as a liquid in PBS, 0.02% sodium azide.
Extra Details:: The mammalian Target of Rapamycin (TOR, also known as mTOR) is an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors. It was initially discovered as a kinase whose ability to stimulate T cell proliferation in response to IL-2 could be inhibited by the immunosuppressive drug rapamycin. Rapamycin inhibits TOR in other cell types resulting in reduced cell growth and reduced rates of cell cycle and cell proliferation. TOR is normally associated with the regulatory proteins RAPTOR and GbetaL. Its downstream targets are thought to be the ribosomal protein S6 kinases and the eukaryotic initiation factor 4E binding proteins (4EBPs). Regulation of these protein families allows TOR to control protein biosynthesis. Applications: Suitable for use in ELISA, Western Blot and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1-2ug/ml Immunocytochemistry: 2ug/ml Optimal dilutions to be determined by the researcher. Positive Control: L1210 Cell Lysate Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice