APOBEC3G, NT (APOBEC-related Cytidine Deaminase, APOBEC-related Protein, APOBEC-related Protein 9, ARCD, ARP9, ARP-9, bK150C2.7, CEM15, CEM-15, dJ494G10.1, DNA dC->dU-editing Enzyme APOBEC-3G, FLJ12740, MDS019, Deoxycytidine Deaminase, A3G)

Size

£655.00

SKU:: 146008
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to 15aa from near the N-terminus of human APOBEC3G.
Format:: Supplied as a liquid in PBS, 0.02% sodium azide.
Extra Details:: The Apoliprotein B mRNA-editing, enzyme-catalytic, polypeptide-like (APOBEC) 3 is a multi-isoform member of the cytidine deaminase family of enzymes that act on monomeric nucleoside and nucleotide substrates. Similar to TRIM5a which targets incoming retroviral capsids, APOBEC3 plays a major role in cellular defense against retroviral infection (2,3) as at least two isoforms, APOBEC3G and to a lesser extent APOBEC3F, can be incorporated HIV-1 virions and induce hypermutation in the newly synthesized viral DNA and thus destabilize the viral genome. This innate mechanism of retroviral resistance is counteracted by the HIV-1 Vif protein by inducing the ubiquitization and degradation of APOBEC3G; a single aa substitution (D128K) blocks APOBEC3G depletion without affecting its inhibitory activity. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 5ug/ml Immunohistochemistry (Formalin fixed paraffin embedded): 1ug/ml Optimal dilutions to be determined by the researcher. Positive Control: Caco-2 Cell Lysate or Human Spleen Tissue Lysate Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice