145818
LRG1, Recombinant, Human (Leucine rich alpha-1-glycoprotein 1, HMFT1766)
Size
£819.00
- SKU:
- 145818
- Molecular Weight:
- 4060
- Purity:
- ≥95% (SDS-PAGE). Endotoxin: ≤0.01EU/ug (LAL).
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Format:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 125ul sterile PBS.
- Extra Details:
- LRG1 (leucinerich alpha2glycoprotein 1) is an ~50kD secreted glycoprotein present in the plasma and other body fluids (14). The human LRG1 cDNA encodes 347 amino acids (aa) that include a 35 aa signal sequence, eight leucinerich repeats (LRR), and an LRRCT (LRR Cterminal) domain (1). Mature human LRG1 shares 66%, 63%, 75% and 72% aa sequence identity with mouse, rat, canine and porcine LRG1, respectively. LRG1 is upregulated during differentiation of neutrophils and present with myeloperoxidase in primary neutrophilic granules (1, 2). It can be produced by hepatocytes as an acute phase protein (5). LRG1 can interact directly with Cytochrome c, and is thought to act as a survival factor for lymphocytes in the presence of extracellular Cytochrome c (3, 6). Serum or plasma LRG1 is reported to be elevated in toxic shock syndrome, cardiac damage (produced by cardiomyocytes and correlating with BNP), autoimmune syndromes such as rheumatoid arthritis and Crohn’s disease, and some cancers such as those in the ovary and biliary tract (4, 710). It is elevated in coronary sinus fluid during ventricular dysfunction and heart failure, and in peritoneal fluid of patients with uterine lyomyomas (8, 11). Recombinant protein corresponding to Val36-Gln347 from human LRG1, fused to 6-his tag at C-terminal, HEK293-derived. Swiss/Uniprot: P02750 Molecular Weight: ~40-60kD Activity: Measured by its binding ability in a functional ELISA. When human Cytochrome c is coated at 1ug/ml (100ul/well), the concentration of recombinant human LRG1 that produces 50% of the optimal binding response is 3-15ng/mL. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice