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145803

HSPA1A, Recombinant, Human (HSP70, HSP70-1A, HSP72, Heat Shock 70kD Protein, Heat Shock 70kD Protein 1A)

Size

£996.00

SKU:
145803
Molecular Weight:
70
Purity:
≥95% (SDS-PAGE)
Storage Conditions:
-70°C
Supplier:
United States Biological
Format:
Supplied as a liquid in HEPES, NaCl, DTT. BSA free.
Extra Details:
Heat Shock 70kD Protein (HSP70), also known as Heat Shock 70kD Protein 1A (HSPA1A), is a 641 amino acid (aa) member of the HSP70 family of molecular chaperones with a predicted molecular weight of 70kD. Human HSP70/HSPA1A shares 95% and 97% aa sequence identity with the mouse and rat orthologs, respectively. It has an N­terminal nucleotide­binding domain, which contains ATPase activity, and a C­terminal substrate­binding domain (1). HSP70/HSPA1A promotes the proper folding of nascent polypeptides and assists in the refolding of denatured proteins (2). However, if either of these processes proceeds too slowly or fails, HSP70/HSPA1A can interact with the HSP40 co­chaperone protein and the CHIP/STUB1 Ubiquitin ligase (E3) to promote ubiquitination and degradation of the nascent polypeptide or denatured protein (3,4). HSP70/HSPA1A can be regulated post­translationally via multiple mechanisms, including phosphorylation, ubiquitination, and methylation (5­8). For example, unmethylated HSP70/HSPA1A localizes to the cytoplasm, but following methylation on Lys561 it is found only in the nucleus (8). Pathologically, HSP70/HSPA1A has been implicated in the promotion of multiple cancer types (8­10). Conversely, it is thought to protect against several neurodegenerative diseases that are caused by the accumulation of misfolded proteins (11,12). Recombinant protein corresponding to Met1-Asp641 from human HSP70/HSPA1A, expressed in E. coli. Molecular Weight: ~70kD Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Shipping Conditions:
Dry Ice