Frizzled-9, Fc Chimera, Recombinant, Mouse, aa24-186 (Frizzled Family Receptor 9, CD349, FZD9)

Size

£1,029.00

SKU:: 145790
Molecular Weight:: 5866
Purity:: ≥95% (SDS-PAGE)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a lyophilized powder in PBS. BSA free. Reconstitute with 100ul sterile PBS.
Extra Details:: Frizzled-9 (Fzd9, previously called Fzd3) belongs to the Frizzled family of seven-transmembrane glycoprotein receptors, which are signaling receptors for the family of secreted Wnt ligands (1-3). Mouse Frizzled-9 cDNA encodes aa592 including a 23aa signal peptide, a 207aa extracellular domain (ECD), the 7TM region (aa231-530), and a C-terminal cytoplasmic domain with a PDZ binding motif. The ECD includes a cysteine-rich region (CRD, aa35-156) that binds Wnts and is highly conserved among Frizzleds. Within aa23-186, mouse Frizzled9 shares 100%, 97%, 97% and 96% aa identity with rat, human, canine and bovine Frizzled9, respectively. The human Frizzled-9 gene is within a large region that is deleted in Williams-Beuren syndrome, a developmental disorder affecting personality and cognition (2). Frizzled-9 mRNA is expressed in the mouse central nervous system and myotomes during development, and persists in the hippocampus throughout life (2-4). Adults also show expression in heart, testis, and skeletal muscle (2-4). Cellular expression is detected in proliferating neural precursors, B lymphocyte progenitors, and differentiating osteoblasts (46). Defects in learning, B lymphocyte development, and bone mineralization are observed in mice deleted for Frizzled-9 (4-6). Wnt-2-stimulated signaling through the canonical pathway using LRP5 as a coreceptor is reported, resulting in beta-catenin stabilization and gene transcription (7). However, the canonical pathway is not involved in bone defects of Frizzled-9-/-mice (6). Signaling through the JNK (noncanonical) pathway is shown when Frizzled9-expressing human non-small cell lung cancer cell lines are engaged by Wnt-7a, promoting activation of PPARgamma and expression of cadherin and Sprouty, and reversing transformation (8-10). Source: Recombinant Fc Chimera protein, a disulfide-linked homodimer, corresponding to Leu24-Asp186 from mouse Frizzled-9 at the N-terminal, Mouse IgG2a, Glu98-Lys330 at C-terminal, expressed in CHO cells. Molecular Weight: ~58-66kD Endotoxin: <0.01EU/1ug (LAL method) Biological Activity: Measured by its ability to bind biotinylated recombinant mouse Wnt-3a in a funtional ELISA with an estimated Kd <10 nM. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice