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145709

Interferon-alpha/beta Receptor 1, Recombinant, Human, aa26-436 (IFNAR1, IFN-alpha/beta R1)

Size

£863.00

SKU:
145709
Molecular Weight:
7090
Purity:
≥95% (SDS­-PAGE)
Storage Conditions:
-20°C
Supplier:
United States Biological
Format:
Supplied as a lyophilized powder in PBS. BSA free. Reconstitute with 500ul sterile PBS.
Extra Details:
Interferon­ alpha/beta receptor 1 (IFN­alpha/beta R1), also known as IFNAR1, is a 100­130kD member of the class II cytokine receptor family of proteins. These proteins form heterodimeric receptor complexes that mediate class II cytokine signals. Subunits of the different receptor complexes are shared and serve multiple functions (1). IFN­alpha/beta R1, in association with IFN­alpha/beta R2, is required for propagating anti­microbial signal transduction triggered by the type 1 interferons such as IFN­alpha and IFN­beta (2, 3). Mature human IFN­alpha/beta R1 consists of a 409 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 100 aa cytoplasmic domain (4). The ECD contains three tandem fibronectin type III repeats and is extensively glycosylated. Within the ECD, human IFN­alpha/beta R1 shares 47% and 50% aa identity with mouse and rat IFN­alpha/beta R1, respectively. Alternative splicing generates two additional isoforms that lack the transmembrane segment and either all or a portion of the cytoplasmic domain. IFN­alpha/beta R1 interacts very weakly or not at all with type 1 interferons and does not stably interact with IFN­alpha/beta R2. Ligands preferentially associate with IFN­alpha/beta R2, and this complex subsequently forms a stable ternary assembly with IFN­alpha/beta R1 (5­7). IFN­alpha/beta R1 also associates with IFN­gamma R2 even in the absence of IFN­gamma stimulation (3). IFN­alpha/beta R1 activation depends on tyrosine phoshorylation as well as palmitoylation of its cytoplasmic domain (8, 9). Rapid down­regulation of the receptor is accomplished by ligand­dependent or ­independent pathways (e.g. VEGF R signaling, TLR signaling, or cellular stress) which induce its serine phosphorylation, ubiquitination, and degradation (10­13). Source: Recombinant protein corresponding to Gly26-Lys436 from human IFN-alpha/beta R1, expressed in NS0 cells. Molecular Weight: ~70-90kD Endotoxin: <0.10EU/1ug (LAL method) Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Shipping Conditions:
Blue Ice