WWTR1 (TAZ, Transcriptional co-Activatorr with PDZ-binding Motif)

Size

£863.00

SKU:: 145656
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to aa267-400 from human TAZ/WWTR1, expressed in E. coli (UniProt # Q9GZV5).
Clone:: 672027
Format:: Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Extra Details:: TAZ (Transcriptional coActivatorr with PDZbinding motif; also WWTR1) is a 5055kD protein that is related to YAP65 TEF1 interacting protein. It is a widely expressed transcriptional coactivator, and should not be confused with tafazzin/Taz, an enzyme associated with lipid metabolism. TAZ influences the nuclear transport of SMAD2, 3 and 4, and in the nucleus, TAZ is known to interact with transcription factors such as TFF1, Pax8, NKX21 and TEADS, serving as a scaffold for transcriptional activation complexes. Human TAZ is 400 amino acids (aa) in length. It contains one WW domain (aa124157) that binds to PPXY motifs, a coiledcoil region (aa225259), and a PDZ binding domain (aa394400). There are at least four utilized phosphorylation sites. When phosphorylated on aa89, TAZ preferentially bind to 1433 proteins, promoting its retention in the cytoplasm. Over aa267400, human TAZ shares 88% aa identity with mouse TAZ. Applications: Suitable for use in ELISA, Western Blot, and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 2ug/ml Immunocytochemistry: 825ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice