VSIG2 (CTH, CTXL, V-set and Ig Domain-containing Protein 2, CTM, CT-like protein)

Size

£767.00

SKU:: 145652
Purity:: Purified by Protein A or G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rat
Immunogen:: Recombinant protein corresponding to aa25-244 from mouse VSIG2, expressed in NS0 cells.
Clone:: 771625
Format:: Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Extra Details:: VSIG2 (V-set and Ig domain-containing protein 2; also CTM and CT-like protein) is presumably a 50‑60 kDa member of the CTX family of Ig-Superfamily proteins. It shows expression in stomach and prostate by Northern blot, and likely participates in cell adhesion. Mouse VSIG2 precursor is 328 amino acids (aa) in length. It is a type I transmembrane (glyco)protein that contains a 220 aa extracellular domain (ECD) (aa 25‑244) and a 63 aa cytoplasmic region. The ECD contains one V-type (aa 25‑138) and one C2-type Ig-like domain (aa 145‑234). Over aa 25‑244, mouse VSIG2 is 94%, 83% and 85% aa identical to rat, canine and human VSIG2, respectively. Two potential splice variants exist, one that shows a deletion of aa 305‑328 and a second that shows a deletion of aa 144‑236. Applications: Suitable for use in ELISA and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry: 8-25ug/ml using perfusion fixed frozen sections of mouse stomach Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice