Vimentin (VIM) [KO Validated]

Size

£889.00

SKU:: 145650
Purity:: Purified by Protein G affinity chromatography from hybridoma culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rat
Immunogen:: Recombinant protein corresponding to Ser2-Glu466 from human Vimentin, expressed in E. coli.
Clone:: 280618
Format:: Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
Extra Details:: Vimentin is a 57kD class III intermediate filament (IF) protein that belongs to the intermediate filament family. It is the predominant IF in cells of mesenchymal origin such as vascular endothelium and blood cells. The human Vimentin cDNA encodes a 466 amino acid (aa) protein that contains head and tail regions with multiple regulatory Ser/Thr phosphorylation sites, and a central rod domain with three coiledcoil regions separated by linkers. Human Vimentin shares 9798% aa identity with mouse, rat, ovine, bovine, and canine Vimentin. Sixteen Vimentin coiledcoil dimers selfassemble to form intermediate (1012nm wide) filaments. These filaments then anneal longitudinally to form nonpolarized fibers that support cell structure and withstand stress. IF fibers are highly dynamic, and halflife depends on the balance between kinase and phosphatase activity. For example, phosphorylation followed by dephosphorylation drives IF disintegration, followed by reorganization during mitosis. Interactions of head and tail domains link IFs with other structures such as actin and microtubule cytoskeletons. Vimentin is involved in positioning autophagosomes, lysosomes and the Golgi complex within the cell. It facilitates cell migration and motility by recycling internalized trailing edge integrins back to the cell surface at the leading edge. Vimentin helps maintain the lipid composition of cellular membranes, and caspase cleavage of Vimentin is a key event in apoptosis. Phosphorylation promotes secretion of Vimentin by TNFalphastimulated macrophages. Extracellular Vimentin has been shown to associate with several microbes, and appears to promote an antimicrobial oxidative burst. Cellassociated Vimentin can also interact with NKp46 to recruit NK cells to tuberculosisinfected monocytes. Applications: Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1-2ug/ml Immunohistochemistry: 0.5-25ug/ml using immersion fixed paraffin-embedded sections of human tonsil Flow Cytometry (Intracellular): 0.25/10e6 cells using A172 human glioblastoma cell line Immunocytochemistry: 8-25ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice