145633
TLR3 (CD283) (APC)
Size
£843.00
- SKU:
- 145633
- Application:
- Flow Cytometry
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Goat
- Immunogen:
- Recombinant protein corresponding to human Toll-Like Receptor 3, NS0-derived (UniProt # O15455).
- Format:
- Supplied as a liquid in saline, 0.5% BSA, 0.09% sodium azide. Labeled with Allophycocyanin (APC).
- Extra Details:
- Human TLR3 is a 116kD type I transmembrane glycoprotein that belongs to the mammalian Toll-Like Receptor family of pathogen pattern recognition molecules.1 TLR3 is found in phagosomes, where the acidic pH enables binding of internalized double-stranded RNA and mRNA from viruses, parasites, and necrotic virally-infected cells.2-6 Ligand binding by TLR3 induces receptor dimerization, leading to the release of inflammatory cytokines and dendritic cell maturation.3, 4, 6, 8 TLR3 is expressed in dendritic cells, macrophages, microglia, and astrocytes, and is upregulated by IFN-beta and LPS.4, 8-10 TLR3 expression is also induced by lung fibroblasts and epithelial cells by respiratory syncytial virus infection.7 The extracellular domain (ECD) of human TLR3 shares 80%, 79%, and 77% aa sequence identity with the ECD of rat, mouse, and bovine TLR3, respectively. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- Shipping Conditions:
- Blue Ice