145626
Thymic Stromal Lymphopoietin Receptor (TSLP R, CRL2, CRLF2, CRLM-2, CRLM2, Cytokine Receptor-like Module 2, PCOR1) (APC)
Size
£793.00
- SKU:
- 145626
- Application:
- Flow Cytometry
- Purity:
- Purified by Protein A or G affinity chromatography.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Goat
- Immunogen:
- Recombinant protein corresponding to aa20-233 from mouse TSLP R, NS0-derived.
- Format:
- Supplied as a liquid in PBS, 0.5% BSA, 0.09% sodium azide. Labeled with Allophycocyanin (APC).
- Extra Details:
- TSLP R, also named Delta and CRLM2 (cytokine receptor-like Module 2), was originally cloned as a novel type 1 cytokine receptor with similarity to the common gamma chain.1, 2 It was subsequently identified to be a subunit of the cellular receptor for the IL-7-like cytokine TSLP and termed TSLP R.3 The TSLP R cDNA encodes a 359 amino acid (aa) residue type I membrane protein with a 24aa residue signal peptide, a 206aa residue extracellular domain that shares 24% sequence identity with the mouse common gamma-chain receptor, a 23aa residue transmembrane domain, and a 106aa residue cytoplasmic domain. The cytoplasmic domain of TSLP R contains a membrane-proximal box1 motif which is known to be important for association with Jaks. An alternatively spliced mRNA variant encoding a soluble TSLP R has also been reported.2 TSLP R expression is ubiquitous in immune and hematopoietic cells, but is upregulated in Th2-skewed cells. Cells expressing TSLP R alone bind TSLP with low affinity. Coexpression of TSLP R and IL-7 R alpha is3, 4 required for high-affinity TSLP binding and signal transduction. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 10ul, 1x10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- Shipping Conditions:
- Blue Ice