TGIF1 (HPE4, 5’-TG[TCA]-3' Interacting Protein 1, Transforming Growth Factor beta-induced Factor, HPE4)

Size

£780.00

SKU:: 145624
Purity:: Purified by Protein A or G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to aa245-359 from human TGIF1, expressed in E. coli (UniProt # Q15583).
Clone:: 790707
Format:: Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Extra Details:: TGIF1 (5'TG[TCA]3' Interacting Protein 1; also Transforming growth factor betainduced factor and HPE4) is a 43kD (predicted) member of the TALE/TGIF homeobox family of proteins. It is widely expressed, and serves as a transcriptional corepressor for SMAD2induced gene activation. Evidence suggests that it either complexes with cjun and cPML, or interacts with HDAC in the nucleus to perform this function. It also binds to RXR response elements, blocking RXRmediated gene activation. Such interactions are of considerable importance during fetal and neonatal development. Human TGIF1 is 401 amino acids (aa) in length. It contains a DNAbinding homeobox domain (aa164226) and two consecutive repressor domains (aa267401). There are multiple splice variants. One is very well characterized, is 3335kD in size, and shows a four aa substitution for aa1133. This is most active when phosphorylated, which adds ~24kD to its SDSPAGE MW. Two other splice forms contain a 19aa substitution for aa1134, and utilize an alternative start site at Met150, respectively. Over aa245359, human TGIF1 shares 81% aa identity with mouse TGIF1. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice