Stabilin-1 (CLEVER-1, FEEL-1, FELE-1, FEX1, MS-1 Antigen, STAB1, Fasciclin, EGF-like, laminin type EGF-like, link domain containing scavenger receptor-1)

Size

£825.00

SKU:: 145616
Purity:: Purified by Protein A affinity chromatography from hybridoma culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to Gly2208-Val2305 from human Stabilin-1, expressed in E. coli.
Clone:: 840449
Format:: Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
Extra Details:: Stabilin1, also known as FEEL1 (fasciclin, EGFlike, laminin type EGFlike, and link domain containing scavenger receptor1) and CLEVER1 (common lymphatic endothelial and vascular endothelial receptor1), was originally identified as the MS1 antigen. It is a type I transmembrane multidomain protein with a large extracellular domain that contains 7 fasciclin domains, multiple EGFlike and laminin type EGFlike domains, and a link domain. The short cytoplasmic tail interacts with cargo adaptors that link cargo into the clathrincoated pits (CGAs). Stabilin1 is expressed by tissue macrophages and sinusoidal endothelial cells, which are scavenging cells. Stabilin1 is involved in intracellular trafficking pathways and binds multiple ligands, including acetylated LDL, SPARC, AGE, and SICLP. An alternatively spliced, soluble, 803 amino acid (aa) Stabilin1 that diverges after aa746 has been identified. Within the region used as immunogen, human Stabilin1 shares 83% and 84% amino acid sequence homology with mouse and canine Stabilin1, respectively. Applications: Suitable for use in ELISA and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry: 8-25ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice