145606
Siglec 10 (Sialic Acid-binding Ig-like Lectin 10, Siglec like Protein 2, SLG2, UNQ477, PRO940)
Size
£825.00
- SKU:
- 145606
- Purity:
- Purified by Protein A or G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- Recombinant protein corresponding to aa20-525 from mouse Siglec-G, CHO-derived (Accession# NP_766488).
- Clone:
- 805903
- Format:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- Siglecs (sialic acid binding Ig-like lectins) are Itype lectins that belong to the immunoglobulin superfamily. They are characterized by a V-type-Iglike domain which mediates sialic acid binding, followed by varying numbers of C2-type Iglike domains. Mouse Siglec-G, the apparent ortholog of human Siglec-10, is a 110-120kD, 688 amino acid (aa) type I transmembrane protein mainly expressed on mouse B1-type B cells. It controls B1 cell survival, selection, expansion and calcium signaling by negatively regulating B cell receptor signals. A potentially secreted 269aa variant diverges after the first two Ig-like domains. Mouse Siglec-G shares ~63% aa sequence identity with human Siglec-10 within the extracellular domain. Applications: Suitable for use in Flow Cytometry and ELISA. Other applications not tested. Recommended Dilution: Flow Cytometry: 2.5ug/10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice