Siglec 11 (Sialic Acid Binding Ig-like lectins)

Size

£825.00

SKU:: 145605
Purity:: Purified by Protein A or G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Synthetic peptide corresponding to Human Siglec-11 (UniProt # Q96RL6).
Clone:: 705904
Format:: Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
Extra Details:: Siglecs (sialic acid binding Iglike lectins) are Itype lectins that belong to the immunoglobulin superfamily. They are characterized by an Nterminal Iglike Vset domain which mediates sialic acid binding, followed by a varying numbers of Iglike C2set domains. Siglecs3 and 513 constitute the CD33/Siglec3 related group, which are defined by their sequence homology and differential expression in the hematopoietic system (13). Mature human Siglec11 consists of a 534 amino acid (aa) extracellular domain (ECD), a 23 aa transmembrane segment, and a 114 aa cytoplasmic domain. The ECD contains one Iglike Vset domain, and three Ig like C2set domains. The cytoplasmic domain contains two immunoreceptor tyrosinebased inhibitory motifs (ITIMs) (4). A splice variant of Siglec11 has a deletion of nearly 100aa in the extracellular juxtamembrane region. Among siglecs, the ECD of Siglec11 is most closely related to that of Siglec10 (82% aa sequence identity). The cytoplasmic domains of these proteins are only 20% identical. Siglec11 is closely related to the pseudogenes Siglec14 and Siglec16 (4, 5). Human Siglec11 shares 90%96% aa sequence identity with Siglec11 from great apes. Rodent orthologs of Siglec11 have not been identified. In human, Siglec11 is expressed in tissue macrophages, brain microglia, and inflammatory site monocytes (4). Strong microglial expression is specific to humans, as it is less prominent or absent in chimpanzees and orangutans (5). Siglec11 forms 180kD disulfidelinked dimers. It shows a strong binding preference for sialic acid in alpha 28 linkage which is unusual for siglecs (4). A conserved arginine in the Iglike Vset domain only partially contributes to Siglec11 ligand recognition, in contrast to its being required in other siglecs (4). Tyrosine phosphorylation of the cytoplasmic region of Siglec11 promotes association with the phosphatases SHP1 and SHP2 (4). Applications: Suitable for use in ELISA and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry: 825ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice