145604
SH2B1 (PSM, Src-Homology 2 Domain-Containing Protein B1/1B, SH2B1alpha, PSM, SH2B Adaptor Protein 1)
Size
£837.00
- SKU:
- 145604
- Application:
- WB
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Goat
- Immunogen:
- Recombinant protein corresponding to aa317-467 from human SH2B1, expressed in E. coli.
- Format:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Extra Details:
- SH2B1 (Src-Homology 2 Domain-Containing protein B1/1B; also SH2B1alpha, PSM and SH2B adaptor protein 1) is a member of the SH2B adapter family of proteins. Although its predicted MW is 80kD, it runs anomalously at 90-102kD in SDS-PAGE. SH2B1 is a nucleocytoplasmic protein that is widely expressed in cells such as skeletal muscle cells, neurons, and adipocytes. It serves as a linker between Jak2 and multiple downstream molecules such as Rac and IRS1. It does this following phosphorylation on a number of potential sites. It also promotes the enzymatic activity of associated receptor kinases, thus potentiating ligand-receptor interactions. SH2B1 appears to bind to nonphosphorylated Jaks as a monomer, while Jak phosphorylation induces SH2B1 dimerization. SH2B1 is further reported to form a homopentamer, and to oligomerize with SH2B2. Human SH2B1(alpha) is 756 amino acids amino acids (aa) in length. It contains one dimerization segment (aa24-85), an NLS (aa224-233), one pleckstrin homology region (aa249-378) and an SH2 domain (aa521-625). At least three utilized phosphorylation sites exist. There are two splice variants for SH2B1. The 90-92kD SH2B1beta isoform shows a 39aa substitution for aa633-756, while the SH2B1gamma isoform possesses a 50aa substitution for the same aa range (i.e., aa633-756). Over aa317-467, human SH2B1 shares 92% aa identity with mouse SH2B1. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice