145602
SEMA4A (Semaphorin 4A, CORD10, SEMAB, SEMB)
Size
£876.00
- SKU:
- 145602
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Sheep
- Immunogen:
- Recombinant protein corresponding to aa32-683 from human Semaphorin 4A, NS0-derived.
- Format:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Extra Details:
- Sema4A (Semaphorin 4A; also Sema B) is a class IV member of the semaphorin family of proteins. It is expressed by dendritic cells, monocytes, T cells, B cells, astrocytes and oligodendrocytes, plus visceral smooth muscle and bronchial epithelial cells. It reportedly binds to Tim2 and Plexin D1 and B1. In the immune system, it serves to activate naïve T cells and induce a Th1 phenotype. In the nervous system, it induces growth cone collapse in neurons. Mature human Sema4A is an 80kD (predicted) type I transmembrane glycoprotein that is 729 amino acids (aa) in length (aa33761). It contains a 651aa extracellular region (aa33683) that is characterized by the presence of one Sema domain (aa 36494), a PSI region (aa496528), and an Iglike C2type domain (aa573631). The 57 aa cytoplasmic region contains two potential phosphorylation sites at Thr744 and Ser747. There are two potential splice variants. One contains a 37aa substitution for aa293328, while a second contains an alternative start site at Met100 coupled to a deletion of aa122154. Sema4A is reportedly cleaved to generate both a monomeric and a dimeric soluble isoforms. Over aa 32683, human Sema4A shares 86% aa identity with mouse Sema4A. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 2ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice