145601
SEMA3C (Semaphorin 3C, SEMAE, Semaphorin E, SEME)
Size
£825.00
- SKU:
- 145601
- Purity:
- Purified by Protein A affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Gly21-Ser738 from human SEMA3C expressed in NS0 cells.
- Clone:
- 757820
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- Semaphorin 3C is one of six Class 3 secreted semaphorins which share 40-50% amino acid (aa) identity. Class 3 semaphorins are potent chemorepellents that function in axon and/or vascular guidance during development, and may be upregulated in tumor progression. The 751 amino acid mouse Sema3C is highly modular. It contains a 20aa signal sequence, an ~500aa Nterminal Sema domain that forms a betapropeller structure similar to that found in integrin molecules, a cysteine knot, a furintype cleavage site, an Iglike domain, and a Cterminal basic domain. Covalent dimerization plus cleavage at the Cterminus are required for activity of class 3 semaphorins. Mouse Sema3C shares at least 95% aa identity with human, rat, cow and dog Sema3C, and 89% and 75% aa identity with chick and zebrafish Sema3C, respectively. Type 3 semaphorins transduce signals through transmembrane plexins, either directly or by binding associated neuropilin receptors. Sema3C signaling is transduced by PlexinD1 indirectly via Neuropilin1 or Neuropilin2 receptors. Sema3C is expressed in all somitic motor neurons, in lung buds and in cardiac neural crest cells during development. Sema3C activates integrins in certain cells so, in addition to its repulsive activities, it sometimes acts as a chemoattractant. In the developing nervous system, this chemoattraction appears to complement Sema3A repulsion in adjacent cell layers. Sema3C also provides an attractive force opposing Sema6A and Sema6B to guide migration of neural crest endothelial cells to the cardiac outflow tract. Consequently, defects in aortic arch formation occur when Sema3C or PlexinD1 genes or Sema3Cneuropilin interactions are disrupted. Applications: Suitable for use in ELISA and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Immunocytochemistry: 8-25ug/ml using immersion fixed MCF-7 human breast cancer cell line. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice