145598
RPS6 (Ribosomal Protein 56, Phosphoprotein NP33)
Size
£863.00
- SKU:
- 145598
- Application:
- WB
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Sheep
- Immunogen:
- Recombinant protein corresponding to aa1-249 from human Ribosomal Protein 56, expressed in E. coli (UniProt # P62753).
- Format:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 1ml sterile PBS.
- Extra Details:
- (40S) Ribosomal protein S6 (rpS6; also Phosphoprotein NP33) is a 3234kD member of the ribsosmal protein S6e family of molecules. It is ubiquitously expressed and involved in the regulation of cell size. Higher eukaryotic ribosomes contain a small (or 40S) and large (or 60S) subunit. The small subunits are composed of one RNA molecule plus more than 30 individual proteins, one of which is rp6S. It is a substrate of both PASKIN and S6 kinase, and when phosphorylated Cterminally, appears to slow the rate of protein synthesis. Notably, the activities of rpS6 are complex, as phosphorylation seems to decrease cell proliferation and glucose utilization on one hand, while increasing cell size on the other. Human ribosomal protein S6 is 249 amino acids (aa) in length. It possesses two acetylated lysines plus six phosphorylated serines. There are three potential splice forms. One contains a four aa substitution for aa146, a second utilizes an alternative start site at Met32, and a third utilizes the same Met32 start site coupled to a substitution of a Gln residue for aa234243. Fulllength human, mouse and rat rpS6 are identical in their amino acid sequences. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice