RAMP2 (Receptor Activity Modifying Protein 2)

Size

£825.00

SKU:: 145593
Purity:: Purified by Protein A or G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rat
Immunogen:: Recombinant protein corresponding to aa45-159 from mouse RAMP2, expressed in E. coli (UniProt # Q9WUP0).
Clone:: 773816
Format:: Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Extra Details:: RAMP2 (receptor activity modifying protein 2) is a 20kD member of the RAMP family of proteins. It is expressed on cardiomyocytes, vascular smooth muscle cells and endothelium and interacts with CRLR to form a receptor complex for adrenomedullin (AM). AM induces vasodilation on AM1 receptor expressing cells. Mature mouse RAMP2 is a 145 amino acid (aa) type I transmembrane glycoprotein that contains a 115aa extracellular domain (ECD) (aa45-159) and a 9aa cytoplasmic region. Although the ECD contains no typical structural motifs, based on human, aa100-106 are critical for AM binding. Over aa45-159, mouse RAMP2 shares 57% and 83% aa identity with human and rat RAMP2, respectively. Applications: Suitable for use in Flow Cytometry, ELISA, and Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 2.5ug/10e6 cells Immunohistochemistry: 825ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice