PSMB9 (LMP2, Large Multifunctional Peptidase 2, Proteasome Subunit beta-1i, RING12)

Size

£825.00

SKU:: 145588
Purity:: Purified by Protein A or G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to aa21-219 from human LMP2/PSMB9 expressed in E. coli (UniProt # P28065).
Clone:: 792520
Format:: Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
Extra Details:: LMP2 (large multifunctional peptidase 2), also called PSMB9 (proteasome subunit beta 9) or PSMB6i, is a 22kD broadspecificity aminopeptidase of the T1B family that is a component of the 20S immunoproteasome core (a7b7b7a7) of the 26S proteasome particle. Induction of LMP2 by IFNg, IRF1, TNFa, CD40L, or heat shock in immuneresponsive cells or cancer cells promotes replacement of proteasome subunit PSMB6 by LMP2 and processing of peptides for presentation by MHC I. After autocleavage between amino acids (aa) 2021, human LMP2 (aa21219) shares 91% aa sequence identity with mouse and rat LMP2. Alternate splicing creates a short form, LMP2.S, which lacks aa413 within the propeptide. Applications: Suitable for use in ELISA, Western Blot, and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml Immunocytochemistry: 825ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice