145580
PROS1 (Protein S)
Size
£1,010.00
- SKU:
- 145580
- Purity:
- Purified by Protein A or G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- Recombinant protein corresponding to aa1-669 from mouse Protein S, CHO-derived (UniProt # Q08761).
- Clone:
- 818002
- Format:
- Supplied as a lyophilized powder in PBS, Trehalose. Reconstitute in sterile PBS.
- Extra Details:
- Protein S (PROS1) is a 7075kD plasma glycoprotein member of the vitamin K dependent family of proteins. It is synthesized by hepatocytes, Sertoli cells, macrophages, vascular smooth muscle cells and endothelium. Protein S is a nonenzymatic cofactor for activated protein C and participates in the degradation of coagulation factors FVa and FVIIIa. It also binds to Axl on smooth muscle, likely initiating mitosis. Protein S circulates free in plasma and, unlike Protein S in human and rat, does not complex with C4BP. Mouse Protein S precursor is 675 amino acids (aa) in length. It contains a signal sequence (aa124), a cleavable prosegment (aa2541) and a 634aa mature region. The mature molecule contains one Gla region (aa4287), four EGFlike domains (aa117283) and two laminin Glike regions (aa299665). Over aa1169 of the mature molecule, mouse Protein S shares 80% and 88% aa identity with human and rat Protein S, respectively. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 2ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice