PRDM3 (EVI-1, Ectopic Virus Integration Site 1, MDS1, MECOM)

Size

£800.00

SKU:: 145579
Purity:: Purified by Protein A or G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to aa241-430 from human EVI-1, expressed in E. coli (UniProt # Q03112).
Clone:: 702321
Format:: Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Extra Details:: Ectopic virus integration site 1 (EVI-1), also known as MECOM, is a 145kD is a transcriptional regulator that interacts with GATA2 and histone methyltransferases. EVI-1 contains 7 tandem N-terminal zinc finger regions (aa21-239), a central region, and a cluster of 3 more zinc fingers (aa733-812). Longer isoforms have 189aa or 64aa N-terminal extensions. EVI-1 target genes are critical to hematopoietic stem cell proliferation and myeloid differentiation. EVI-1 is overexpressed in acute myelogenous leukemia (AML) as well as ovarian cancer. Chromosomal translocations fuse EVI-1 with RUNX1 and RPN1 contribute to chromosomal instability, myeloid leukemia proliferation, and a block in myeloid differentiation. Within aa241-430, human EVI-1 shares 94% aa sequence identity with mouse and rat EVI-1. Applications: Suitable for use in ELISA and Immunocytochemistry. Other applications not tested. Recommended Dilution: Immunocytochemistry: 825ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice