145572
Plexin B2 (MM1, PLEXB2, PLXNB2)
Size
£761.00
- SKU:
- 145572
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to aa20-1160 from human Plexin B2, CHO-derived.
- Clone:
- 537219
- Format:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- Plexin B2 is a 240kD member of the B subfamily, plexin family of semaphorin receptors. It is expressed by cerebellar granule cells, neuroepithelium and chondrocytes, and serves as a receptor for Semaphorin 4C. Mature human Plexin B2 is 1819 amino acid (aa) in length. It is a type I transmembrane (TM) glycoprotein that contains an 1178aa extracellular domain (ECD) plus a 620 aa cytoplasmic region. The ECD contains one semaphorin domain (aa20466) and three IPT repeats (aa8031092). The ECD may be cleaved into two subunits, a 170kD alphachain (aa201164) and an 80kD TM betachain that remain noncovalentlylinked. Multiple splice variants may exist. One shows an alternate start site at Met1369, while a second shows a deletion of aa67638. Over aa201160, human Plexin B2 is 82% aa identical to mouse Plexin B2. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 2ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice