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145570

PIP5K3 (PIKFyve, FAB1, P235, PIP5K, PIPkin-III, PhosphoInositide Kinase for Five Position containing a fyve finger, YOTB, Vac1, EEA1)

Size

£869.00

SKU:
145570
Purity:
Purified by immunoaffinity chromatography.
Storage Conditions:
-20°C
Supplier:
United States Biological
Host:
Sheep
Immunogen:
Recombinant protein corresponding to aa215-361 from human PIP5K3, expressed in E. coli (UniProt # Q9Y2I7).
Format:
Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Extra Details:
PIKfyve (PhosphoInositide Kinase for five position containing a fyve finger (FAB1/YOTB/Vac1/EEA1) domain; also phosphatidylinositol 3­phosphate 5­kinase type III and Type III PIP kinase) is a 230­260kD cytoplasmic and endocytic member of an ancient family of phosphatidylinositol­3,5 biphosphate (PI[3,5]P2 synthesizing enzymes. It is associated with endosomes, particularly the microdomains of early endosomes that are EEA1­deficient. PIKfyve binds to membrane­embedded phosphatidylinositol and initially converts PI3P into PI(3,5)P2, which is then converted into PI5P. In conjunction with ArPIKfyve and Sac3, its actions are involved in the normal transport, fusion, and membrane export of endosomes. Notably, PIKfyve also appears to act as a protein kinase, apparently controlling its own activity through autophosphorylation. Human PIKfyve is 2098 amino acids (aa) in length. It contains one FYVE PI3P­binding zinc­finger domain (aa154­219), a DEP domain (aa365­440) and a C­terminal PIP kinase rehion (aa1172­2085). There are no less than 29 utilized Ser/Thr phosphorylation sites, plus two utilized Tyr phosphorylation sites. Three potential isoform variants are reported, all which possess a three aa substitution for aa546­2098. Two of these three also demonstrate a deletion of aa108­204, and a nine aa substitution for aa109­203, respectively. Over aa215­361, human and mouse share 95% aa sequence identity. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.25ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Shipping Conditions:
Blue Ice