PFKM (GSD7, PFK-1, PFK-A, PFKX, Phosphofructokinase-1, Phosphofructokinase-M, Phosphohexokinase)

Size

£825.00

SKU:: 145567
Purity:: Purified by Protein A or G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to aa674-780 from human PFKM, expressed in E. coli (UniProt # P08237).
Clone:: 842735
Format:: Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
Extra Details:: PFKM (PhosphoFructoKinase, Muscle type; also PFKA and Phosphohexokinase)) is a 8586kD member of the phosphofructokinase family, two domain subfamily of enzymes. It is one of three functionally related enzymes, all expressed by distinct genes. While PFKM is expressed by virtually all cells, its name is based on the fact that skeletal muscle expresses only the PFKM isotype. PFK catalyzes the essentially irreversible phosphorylation of Fru6P, forming Fru1,6bisP. This determines the rate of cellular glycolysis. As a monomer, PFKM is unstable, and following synthesis, PFKM forms low activity homodimers. High activity comes with tetramerization/oligomerization. In all cases, multimerization is associated with PFKM interaction with various components of the cell cytoskeleton. Human PFKM is 780 amino acids (aa) in length. It contains a 740aa catalytic region (aa16755) plus one utilized phosphorylation site at Thr2. There are at least two potential isoform variants. One contains an alternative start site 71aa upstream of the standard site, while another shows a deletion of aa282312. Proteolysis of PKFM apparently occurs, generating 4547kD fragments. Over aa674780, human PFKM shares 98% aa sequence identity with mouse PFKM. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.2ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice